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. 2009 May 4;185(3):503–519. doi: 10.1083/jcb.200809085

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© 2009 Lapetina et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Arg-YFP and cortactin-RFP colocalize to membrane protrusions in response to adhesion to fibronectin. Individual frames from time-lapse videos of arg^−/− cells expressing Arg-YFP and cortactin-RFP (Video 1). (A–F) Phase-contrast (A and D), YFP channel (B and E), and RFP channel (C and F) images are shown. (D–F) Enlargements of the regions boxed in A, showing colocalization of Arg-YFP and cortactin-RFP (indicated by arrowheads) followed by the formation of a cell edge protrusion (indicated by asterisks). The elapsed time is shown in minutes:seconds. Bars, 10 µm.