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. 2009 May 4;185(3):503–519. doi: 10.1083/jcb.200809085

Figure 5.

Figure 5.

A Pro-rich region in the Arg C terminus is required for cell edge dynamics. (A) Graphical depiction of Arg mutants used in this experiment. See Fig. 3 for a description of the domains. KI, KI (K317M) point mutation in the active site. Arg mutSH2 contains a point mutation (R176K) in the SH2 domain that abrogates binding to phospho-Tyr–containing binding partners. Arg 557-C PXXP mut1 and Arg 557-C PXXP mut23 contain point mutations in PXXP motif 1, and PXXP motifs 2 and 3, respectively. (B–J) Representative images of 10-min time-lapse videos of cells spreading on glass coverslips coated with fibronectin. The three rightmost panels are representative kymographs for each genotype. Kymographs were constructed as in Fig. 2. WT cells (n = 20 cells; B) and arg−/− cells expressing YFP (n = 22 cells; C), Arg-YFP (n = 34; D), KI Arg (n = 29 cells; E), Arg mutSH2 (n = 20 cells; F), Arg C terminus 557-C (n = 29 cells; G), Arg 688-C (n = 17 cells; H), Arg 557-C PXXP mut1 (n = 22 cells; I), and Arg 557-C PXXP mut23 (n = 16 cells; J) are shown. (K and L) Quantification of cell edge dynamics. Mean ± SEM. ANOVA between all cell types: protrusions, P < 0.0001; retractions, P < 0.0001. Fisher’s PLSD for all cells versus arg−/−: *, P < 0.01. Bars, 10 µm.