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. 2009 May 4;185(3):503–519. doi: 10.1083/jcb.200809085

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© 2009 Lapetina et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Either Abl or Arg kinase activity is sufficient for protrusions, but Arg uniquely provides a scaffold function required for cell edge protrusion. (A and B) Quantification of protrusion (A) and retraction (B) data for WT cells treated with DMSO vehicle or 10 µM STI-571 and Abl/Arg dko cells expressing YFP, Arg-YFP, KI Arg-YFP (Arg KI), or Abl-YFP. Mean ± SEM. Fisher’s PLSD of WT versus WT + STI-571: *, P < 0.0001. (C–H) Representative frames from 10-min time-lapse videos of cells plated on glass coverslips coated with 10 µg/ml fibronectin and treated with DMSO vehicle (n = 20 cells; C), 10 µM STI-571 (n = 20 cells; D), and dko cells expressing YFP (n = 20 cells; E), Arg-YFP (n = 16 cells; F), Arg KI-YFP (n = 20 cells; G), or Abl-YFP (n = 30; H) are shown. The three rightmost panels are representative kymographs for each genotype. Kymographs were constructed as in Fig. 2. Bars, 10 µm.