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. 2009 May 4;185(3):475–491. doi: 10.1083/jcb.200810030

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© 2009 Onischenko et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — The role of Pom152, Pom34, Nup53, and Nup59 in targeting of Ndc1 to NE. (A) Localization of Ndc1 was compared in nup53Δ pom34Δ pom152Δ MET3-3×HA-NUP59 (i), nup53Δ MET3-3×HA-NUP59 (ii), pom34Δ pom152Δ GAL-3×HA-NUP59 (iii), and GAL-3×HA-NUP59 (iv) cells by fluorescence microscopy (via the yeast EGFP tag in i and ii) or by indirect immunofluorescence (via 13×myc tag in iii and iv) in permissive (galactose medium [gal] or medium lacking methionine [−met]) and nonpermissive conditions (dextrose-containing medium [dex] or medium containing 10× methionine [+10× met]). For immunofluorescence, cells were also stained with DAPI to label DNA. (B) Similar analysis of Ndc1–yeast EGFP localization in nup53Δ nup59Δ GAL-3×HA-POM34 (i), GAL-3×HA-POM34 (ii), nup53Δ nup59Δ GAL-3×HA-POM152 (iii), and GAL-3×HA-POM152 (iv). Bars, 5 µm.