Table 1.
Loss of drug resistance, aFGF, and bFGF upon disaggregation of metastatic tumors and upon passaging in monolayer cultures
| Culture conditions | Concentration producing 50% inhibition
|
Amount in CM, pg/ml
|
|||
|---|---|---|---|---|---|
| Pac, nM | Dox, nM | 5-FU, μM | aFGF | bFGF | |
| Histocultures* | |||||
| Primary | >14 | 18 | 33 | 62 | 130 |
| Lymph node | >227† | 165† | NM | 195† | 660† |
| Lung | >1,804† | NM | 122† | 269† | 845† |
| Monolayers | |||||
| (passage 0) | |||||
| Primary | 10.7 | 8.0 | 0.79 | 62 | 93 |
| Lymph node | 32.9† | 31.0† | 1.90† | 100† | 243† |
| Lung | 92.4† | 77.0† | 2.84† | 118† | 302† |
| Monolayers | |||||
| (passage 1) | |||||
| Primary | 10.5 | 8.6 | 0.85 | NM | NM |
| Lymph node | 21.8† | 17.9† | 1.29† | NM | NM |
| Lung | 28.1† | 31.7† | 1.52† | NM | NM |
| Monolayers | |||||
| (passage 2) | |||||
| Primary | 10.3 | 8.0 | 0.79 | NM | NM |
| Lymph node | 15.0† | 11.0† | 1.09† | NM | NM |
| Lung | 21.5† | 16.6† | 1.20† | NM | NM |
| Monolayers | |||||
| (passage 3) | |||||
| Primary | 10.0 | 8.0 | 0.78 | 54 | 95 |
| Lymph node | 11.7 | 7.8 | 0.83 | 51 | 89 |
| Lung | 11.4 | 8.0 | 0.81 | 58 | 103 |
Paired rat primary and metastatic tumors were cultured as histocultures or monolayers. For histocultures, tumors were treated with paclitaxel (Pac) for 24 h (12 pairs of primary and lymph node tumors and 2 pairs of primary and lung tumors), and with doxorubicin (Dox) (3 pairs of primary and lymph node tumors) and 5-fluorouracil (5-FU) (2 pairs of primary and lung tumors) for 96 h. For monolayers, cells were treated with drugs for 96 h, and drug effect was measured by the BrdUrd incorporation method. Mean values. NM, not measured.
In histocultures, Pac at 10,000 nM produced <50% inhibition; data shown are the concentrations that produced 30% inhibition.
P < 0.05 for differences between primary and metastatic tumors (unpaired Student's t test).