. 2003 Oct 16;3:8. doi: 10.1186/1472-6785-3-8

Table 1.

Key to grass identification at Sourhope using trnL PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trnL PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

trnL PCR	Restriction digest 1	Restriction digest 2	Identity (accession number)
~500 bp	BfaI: 158/277/61		Agrostis capillaris (AY177347^b)
	BfaI: 439/61		Agrostis vinealis (AY177332)
>500 bp	NlaIV/DdeI: 57/53/187/317-329		Poa pratensis/trivialis^a(AY177349^b/AY177338)
	NlaIV/DdeI: 57/53/200-204/298-300	XmnI: 197/45/367-370	Festuca rubra/ovina^a (AY177345^b/AY177342)
		XmnI: 197/45/178/154	Festuca juncifolia (AY177344)
	NlaIV/DdeI: 57/53/505-536	Hsp92II: 125/435/86	Deschampsia flexuosa (AY177336)
		Hsp92II: 125/405/86	Deschampsia caespitosa (AY177335)
		Hsp92II: 336/200/86	Nardus stricta (AY177337)
		Hsp92II: 563/86	Holcus lanatus (AY177348)
		Hsp92II: 343/281	Luzula campestris (AY177333)
		Hsp92II: 528/86	Phleum pratense (AY177339)

^acan be separated by ITS PCR-RFLP with HinfI (Pp:335/293/76/17, Pt:293/207/127/76/17; Fr:337/297/76/17, Fo:293/230/107/76/17) ^bsequences from reference [6].