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Efficiency and linearity of the Power SYBR® Green RNA-to-CT™ 2-Step kit run as one-step reaction. PCR amplification of standard curves generated using GAPDH as a reference gene on BR (A) and AB machines (B) using total RNA in amounts ranging from 500 ng to 3.2 pg (fivefold serial dilutions). (Insets) Graphs show the amplification plot of the standard curves, which were run on BR and on AB, respectively.
