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. 2009 Apr 6;185(1):163–176. doi: 10.1083/jcb.200806019

Table I.

Summary of translocation data

Cell type Primary cilium PDGF-AA EIPA Translocation
µm
NIH3T3 No (interphase) 60 ± 8.7 (17)
NIH3T3 No (interphase) + 30 ± 3.0 (25)
NIH3T3 No (interphase) + 60 ± 5.4 (26)
NIH3T3 No (interphase) + + 33 ± 2.7 (25)
NIH3T3 Yes 21 ± 3.6 (23)
NIH3T3 Yes + 11± 1.8 (23)
NIH3T3 Yes + 45 ± 5.7 (25)
NIH3T3 Yes + + 13 ± 1.5 (26)
WT MEF Yes 45 ± 4.2 (53)
WT MEF Yes + 27 ± 4.4 (22)
WT MEF Yes + 111 ± 5.4 (23)
WT MEF Yes + + 15 ± 1.8 (20)
Tg737orpk MEF No or very short 75 ± 5.7 (21)
Tg737orpk MEF No or very short + 63 ± 5.7 (19)
Tg737orpk MEF No or very short + 87 ± 7.8 (34)
Tg737orpk MEF No or very short + + 57 ± 5.7 (30)

The table summarizes the translocation data obtained for NIH3T3 cells, WT MEFs, and Tg737orpk MEFs in 5-h wound-healing assays as a function of (a) the presence or absence of a primary cilium, (b) the presence or absence of 50 ng/ml PDGF-AA, and (c) the presence or absence of 10 µM of the NHE1 inhibitor EIPA. The absence or presence of primary cilia was assessed by visual inspection and has also been previously described (Schneider et al., 2005). Translocation data are shown as mean ± SEM , with the number of cells shown in parenthesis.