Figure 5.

SCaBP8^S237 Phosphorylation Is Required for SCaBP8 Function in Salt Tolerance.

Growth of scabp8 expressing SCaBP8^S237D and SCaBP8^S237A. Five-day-old wild-type, transgenic scabp8 plants containing SCaBP8^S237D or SCaBP8^S237A and scabp8 mutant seedlings were transferred onto Murashige and Skoog (MS) medium ([A] and [D]), MS medium containing 50 mM NaCl ([B] and [E]), or 100 mM NaCl ([C] and [F]). Shoot fresh weight ([G] and [J]), primary root growth ([H] and [K]), and root fresh weight ([I] and [L]) were measured 10 d after transfer. Open bars, wild type; gray bars, SCaBP8^S237D transgenic plants; light-blue bars, SCaBP8^S237A transgenic plants; black bars, scabp8. All data represent means ± se of at least three replicate experiments. Asterisks indicate significant difference between the transgenic line and the wild type or between the transgenic line and scabp8 (Student's t test, *P < 0.05 and **P < 0.01).