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. 2009 May;21(5):1373–1393. doi: 10.1105/tpc.109.065482

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Copyright © 2009, American Society of Plant Biologists

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Figure 6. — NGA Genes Exhibit a Strong but Facultative Response to STY1 Activity.

(A) CRC>>STY1 flower with a severely affected gynoecium, whereas other floral organs are largely unaffected. The apical stigmatic papillae are reduced in their growth (arrow).

(B) Scanning electron micrograph of the CRC>>STY1 gynoecium surface. The epidermis is composed of small cells without significant cuticular outgrowths and numerous intervening immature stomates.

(C) Transverse sections through the CRC>>STY1 gynoecium at the positions marked by arrows in (B). No tissues with the characteristic histology of the mature wild-type gynoecium are apparent, although a rudimentary medial ridge (arrow) is observed.

(D) Expression of nga4:GUS in CRC>>STY1 gynoecium is reduced compared with the wild-type (L).

(E) Gynoecium of a plant cotransactivating STY1 and amiR-NGA^164a by the CRC promoter (CRC>>STY1/amiR-NGA^164a). Stigmatic papillae are lacking (arrow), but the abnormal histology of the epidermal surface appears largely unchanged (F).

(G) to (I) Scanning electron micrographs of a gynoecium with magnified views of the epidermis marked by the arrows.

(G) In the wild type, the style and ovary have distinct cuticular morphologies.

(H) In CRCw>>STY1 gynoecia, the entire ovary surface has style-like epidermal characteristics.

(I) The style-like epidermal features are abolished in CRCw>>STY1/amiR-NGA^164a gynoecium, but it does not revert to the wild type.

(J) and (K) Expression of CRCw>>GUS in a stage 12 flower (J) is apparent in the ovary and nectaries, whereas in a stage 14 flower (K), it is mostly in the nectaries.

(L) to (P) Expression of nga4:GUS in different genotypes.

(L) In the wild-type gynoecium, nga4:GUS is restricted to the style.

(M) In CRCw>>STY1 stage 12, staining is observed in the ovary and nectaries (arrows).

(N) By stage 14-15, CRCw>>STY1 flower staining is restricted to the gynoecium base and nectaries.

(O) and (P) In CRCw>>NGA1 gynoecia (O) or CRC>>NGA1 flowers (P), nga4:GUS staining is not observed in the style-converted gynoecium nor in the papilliae that develop at nectary positions.

(Q) to (U) The effects of ectopic STY1 expression outside the gynoecium.

(Q) A diminutive AP3>>STY1 flower showing reduced growth of all organs.

(R) AP3s>>STY1 flower. Stamen and petal growth is abnormal, and stigma and style tissue is observed on the staminoid organs.

(S) SHP1:GUS is ectopically activated in the carpelloid stamens of AP3s>>STY1 flowers (arrow).

(T) AP3s>>STY1/amiR-NGA^164a flower with comparatively normal stamens.

(U) Ectopic SHP1:GUS is not observed in the third whorl organs of the AP3s>>STY1/amiR-NGA^164a flower.

Bars = 100 μm in (A), (D), (E), (J) to (P), and (R) to (U), 50 μm in (B), (C), and (F), 200 μm in lower-magnification images of (G) to (I), and 20 μm in the adjacent higher-magnification images.