Figure 6.

Competitive Gel-Shift Assay.

DNA fragments containing the GAMYB binding-like motifs of six GA-upregulated genes inhibited the interaction between GAMYB and the ³²P-labeled RAmy1A probe (−380 to −85). Competition experiments were performed using increasing molar amounts (×25, ×50, and ×100) of the indicated unlabeled fragment; lanes 3 to 5, RAmy1A (−380 to −85); lanes 6 to 8, mutated RAmy1A (mRAmy1A) (−380 to −85); lanes 9 to 11, Lipid transporter (−501 to −250); lanes 12 to 14, Male Sterility 2 (−425 to −123); lanes 15 to 17, CYP703A3 (−510 to +40); lanes 18 to 20, KAR (−332 to −4); Lanes 21 to 23, Aspartic protease (−495 to −126); and Lanes 24 to 26, Meiotic serine protease (−397 to −142). Numbers in parentheses indicate the distance in base pairs from the transcriptional initiation site.