Table 2.
FRAP Parameters Estimated for Different Nuclear Fractions of GFP-eIF4A-III Using Curve-Fitting Software TableCurve2D
Hypocotyl Cells
|
Root Cells
|
||||||
---|---|---|---|---|---|---|---|
Culture Cells
|
Control
|
Hypoxia
|
Control
|
Hypoxia
|
|||
Parameter | Nucleoplasm | Nucleolus | Speckle | Nucleoplasm | Speckle | Nucleoplasm | Speckle |
a, Max recovery, decimal fraction ± se | 0.863 | 0.873 | 0.799 | 0.842 | 0.767 | 0.820 | 0.837 |
0.000 | 0.001 | 0.002 | 0.002 | 0.002 | 0.002 | 0.003 | |
b, rate constant, s−1 ± se | 1.860 | 0.450 | 0.240 | 1.701 | 0.535 | 1.462 | 0.432 |
0.110 | 0.020 | 0.008 | 0.092 | 0.019 | 0.087 | 0.016 | |
T1/2, recovery half -time, s | 0.373 | 1.540 | 2.892 | 0.407 | 1.295 | 0.474 | 1.604 |
R2, correlation coefficient | 0.892 | 0.889 | 0.936 | 0.859 | 0.917 | 0.817 | 0.919 |