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. Author manuscript; available in PMC: 2009 Jun 23.
Published in final edited form as: Cell Microbiol. 2007 Nov 2;10(2):514–528. doi: 10.1111/j.1462-5822.2007.01066.x

Figure 2. Defective intracellular growth in strains lacking PC.

Figure 2

(A). Defective growth of ΔpcsA::GmR in bone marrow-derived macrophages. Assays for viable counts over time performed as described (Experimental Procedures). Strains are: GL233, pcsA+pmtA+; CCL24, ΔpcsA; CCL17, ΔpcsA/ppcsA+; LP03, dotA−. Data shown are means ± S.D. of triplicate incubations performed in parallel. (B). Loss of pmtA is not sufficient to impair L. pneumophila intracellular growth. Strains are: CCL25, ΔpmtA; CCL26, ΔpmtA Δpcs; CCL39, ΔpmtA ΔpcsA/ ppcsA+; CCL41, ΔpmtA ΔpcsA / ppmtA+. Data shown are means ± S.D. of triplicate incubations performed in parallel. (C) Absence of PcsA results in defective replication vacuole formation. Macrophages plated on coverslips were incubated with pcsA+ (GL233), pcsA− (GL263) and pcsA−/ppcsA+ (GL266) for 18 hours and processed for immunofluorescence (Experimental procedures). The number of bacteria per macrophages was recorded for 104 phagosomes in triplicate samples for each strain. Shown are typical experiments. All experiments were repeated at least twice.