Table 1.
Bacterial strains and plasmids used in this work.
| Plasmid / Strain |
Genotype/ Relevant Characteristics | Reference |
|---|---|---|
| PLASMIDS | ||
| pDOT1 | pMMB66EH tdΔi bla+ dotA+ | (Berger and Isberg, 1993) |
| pSR47s | KanRsacB suicide vector | (Merriam et al., 1997) |
| pGC36 | pKB5 dotA+ | (Conover et al., 2003) |
| pGC41 | pGC36 pcsA+ | This study |
| pTC5 | pGC36 pmtA+ | This study |
| pTC6 | pSR47s ΔpcsA::GmR | This study |
| pGC51 | pSR47s ΔpmtA | This study |
| pKB5 | pMMB66EH ori RSF1010 tdΔi bla+ | (Berger and Isberg, 1993) |
| STRAINS 1 | ||
| LP02 | Philadelphia-1 rpslL Δ[hsdR-lvh] thyA- | (Berger and Isberg, 1993) |
| LP03 | LP02 dotA03- thyA- | (Berger and Isberg, 1993) |
| JR32 | Philadelphia-1 hsdR | (Sadosky et al., 1993) |
| GL83 | LP02dotA / pKB5 | (Conover et al., 2003) |
| GL84 | LP02dotA / pDOT1 | (Conover et al., 2003) |
| GL233 | LP02dotA / pGC36 | (Conover et al., 2003) |
| GL263 | LP02 pcsA::miniTn10kan dotA/pGC36 | This study, NT |
| GL266 | LP02 pcsA::miniTn10kan dot /pGC41 | This study, NT |
| GL301 | LP02 ΔpmtA dotA- | This study |
| GL302 | LP02 ΔpmtA dotA / pGC36 | This study |
| GL303 | LP02 ΔpmtA thyA- | This study |
| GC197 | LP02 pcsA::miniTn10kan dotA | (Conover et al., 2003) |
| CCL1 | LP02 ΔpmtA dotA thyA- | This study |
| CCL17 | LP02 ΔpcsA::GmR dotA-/ pGC41 | This study |
| CCL21 | LP02 ΔpmtA ΔpcsA::GmR dotA- | This study |
| CCL24 | LP02 ΔpcsA::GmR dotA-/ pGC36 | This study |
| CCL25 | LP02 ΔpmtA dotA-/ pGC36 | This study |
| CCL26 | LP02 ΔpmtA ΔpcsA::GmR dotA- / pGC36 |
This study |
| CCL29 | LP02/pGC41 | This study |
| CCL39 | LP02 ΔpmtA ΔpcsA::GmR dotA-/ pGC41(dotA+ pcsA+) |
This study |
| CCL41 | LP02 ΔpmtA ΔpcsA::GmR dotA-/ pTC5(dotA+ pmt+) |
This study |
1
All strains are derivatives of L. pneumophila Philadelphia 1