TABLE 1.
Semi-quantitative histopathologic analysis of spleen and liver from Strain 13 guinea pigs 9 days after infection with Junin virus
| Histopathology description/score† | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Experimental description* | Spleen | Liver | |||||||
| Junin virus | Dose (PFU/animal) | Guinea pig no. | FN | AI | WPN | PI | LI | N | S |
| Romero | 5.0 × 103 | GP1 | 0 | 2 | 1 | 0 | 2 | 0 | 1 |
| GP2 | 0 | 1 | 1 | 2 | 1 | 1 | 1 | ||
| GP3 | 0 | 2 | 1 | 1 | 1 | 0 | 1 | ||
| 0 | 2 | 1 | 1 | 1 | 0 | 1 | |||
| XJ | 1.5 × 103 | GP12 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
| GP13 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | ||
| GP14 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | ||
| 0 | 1 | 0 | 0 | 1 | 0 | 0 | |||
| – | – | GP15 | 0 | 1 | 0 | 1 | 2 | 0 | 0 |
| GP16 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | ||
| 0 | 0.5 | 0 | 0.5 | 1.5 | 0 | 0 | |||
Eight- to twenty-week-old female Strain 13 guinea pigs were infected with the indicatedstrain and dose (in plaque-forming units [PFU]) of Junin virus by the intraperitoneal route,or as a control, age- and sex-matched Strain 13 guinea pigs were mock-infected (indicated by−) by the intraperitoneal route. Randomly pre-selected animals were humanely killed at9-days post-infection, and the spleen and liver were harvested for histopathologic analysis,performed as described in the Materials and Methods.
FN = fibrinoid necrosis; AI = acute inflammation; WPN = white pulp necrosis; PI =portal inflammation; LI = lobular inflammation; N = necrosis; S = steatosis; 0 = none; 1= mild, 2 = moderate; 3 = severe. Tabulated score is indicated on the bottom row.