TABLE 3.

Semi-quantitative histopathologic analysis of spleen and liver from Junin Romero virus strain–infected Strain 13 guinea pigs at the time of development of severe neurologic symptoms

			Histopathologic description/score†
Experimental description*			Spleen			Liver
Dose (PFU/animal)	Organ harvest (day of study)	Guinea pig no.	FN	AI	WPN	PI	LI	N	S
1.50 × 103	9 dpi	GP15	0	1	2	1	1	0	2
1.50 × 103	12 dpi	GP17	1	0	2	1	1	0	2
2.50 × 103	13 dpi	GP20	NA	NA	NA	0	1	0	2

^*Eight- to twenty-week-old female Strain 13 guinea pigs were infected with the indicated dose (in plaque-forming units [PFU]) of Junin Romero strain virus by the intraperitoneal route. Animals were humanely killed at the time of development of severe neurologic symptoms. The spleen and liver were harvested at the indicated day post-infection (dpi) for histopathologic analysis, performed as described in the Materials and Methods. The survival curves for animals that were followed-up throughout the study period of 19 days are shown in Figure 1B, panel i.

^†FN = fibrinoid necrosis; AI = acute inflammation; WPN = white pulp necrosis; PI = portal inflammation; LI = lobular inflammation; N = necrosis; S = steatosis; 0 = none; 1 = mild, 2 = moderate; NA = not available.