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. Author manuscript; available in PMC: 2009 Jun 23.
Published in final edited form as: Methods Mol Biol. 2008;459:117–130. doi: 10.1007/978-1-59745-234-2_9

Figure 1.

Figure 1

Silver stain analysis of purified PrPC substrate. Twelve percent SDS-PAGE showing (from L to R): (1) Page-ruler prestained protein ladder (Fermentas, Hanover, MD catalog #SM0671) (estimated molecular weights = 170, 130, 100, 72, 55, 40, 33, 24, and 17 kDa); (2) eluate from 3F4 immunoaffinity column; (3) flow through fraction of SP-Sepharose column; and (4–7) successive 2 ml eluate samples from SP-Sepharose column. All chromatographic fractions (lanes 2–7) were concentrated prior to electrophoresis by the method of Wessel and Flugge (14)