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Western blot showing PrPSc amplification. Samples (from L to R): (1) reaction not subjected to proteinase K digestion, showing input PrPC level; (2) time zero (frozen) reaction showing input PrPSc level; (3) amplification reaction without RNA; (4) amplification reaction with poly(A) RNA. Samples 2–4 were subjected to proteinase K digestion.
