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Transfection of siRNA against TLK1 mRNA results in slow progression from G1. Cells were transfected with duplex siRNA oligos, control (scrambled sequence: panel A and B) or TLK1 (panel C and D). Forty-eight hours after transfection, cells were treated (panel B and D), or not, with 1 μM nocodazole for 18 h. The inserted panel E is a western blot of TLK1 with and without treatment with siRNA.
