Table 1.

Primer characteristics used in this study

Sr. number	Product length	Primer name	Locus	Primer sequence
1	220 bp	AZFa	AZFa (prox-2)	5-GGTTCCTGAACAGGGGACT-3
				5-GGCAGCAGAAGGGCCTCTC-3
2	209 bp	Y81	AZFa start	5-AGGCACTGGTCAGAATGAAG-3
				5-AATGGAAAATACAGCTCCCC-3
3	495 bp	ZFY	Control	5′-ACCRCTGTACTGACTGTGATTACAC -3′
				5′-GCACYTCTTTGGTATCYGAGAAAGT -3′
4	320 bp	sY86	AZFa	5-GTGACACACAGACTATGCTTC-3′
				5′-ACACACAGAGGGACAACCCT-3
5	274 bp	sY127	AZFb	5′-GGCTCACAAACGAAAAGAAA-3′
				5′-CTGCAGGCAGTAATAAGGGA-3′
6	350 bp	sY254	AZFc	5′-GGGTGTTACCAGAAGGCAAA-3′
				5′-GAACCGTATCTACCAAAGCAGC-3′
7	326 bp	sY84	AZFa	5′-AGAAGGGTCTGAAAGCAGGT-3′
				5′-GCCTACTACCTGGAGGCTTC-3′
8	301 bp	sY134	AZFb	5′-GTCTGCCTCACCATAAAACG-3′
				5′-ACCACTGCCAAAACTTTCAA-3′
9	126 bp	sY255	AZFc	5′-GTTACAGGATTCGGCGTGAT-3′
				5′-CTCGTCATGTGCAGCCAC-3′
10	249 bp	USP9Y	AZFa	5′-CTTCACACAAATGCGTTTCA-3′
				5′-TGCAATTATTTGAACAAACATGA-3′
11	311 bp	Y143	AZFb	5′-GCAGGATGAGAAGCAGGTAG-3′
				5′-CCGTGTGCTGGAGACTAATC-3′
12	139 bp	Y153	AZFd	5′-GCATCCTCATTTTATGTCCA-3′
				5′-CAACCCAAAAGCACTGAGTA-3′
13	285 bp	Y157	AZFc	5′-CTTAGGAAAAAGTGAAGCCG-3′
				5′-CCTGCTGTCAGCAAGATACA-3′
14	173 bp	Y130	AZFa	5′-AGAGAGTTTTCTAACAGGGCG-3′
				5′-TGGGAATCACTTTTGCAACT-3′
15	177 bp	Y133	AZFb	5′-ATTTCTCTGCCCTTCACCAG-3′
				5′-TGATGATTGCCTAAAGGGAA-3′
16	190 bp	Y121	AZFa/b	5′-AGTTCACAGAATGGAGCCTG-3′
				5′-CCTGTGACTCCAGTTTGGTC-3′
17	109 bp	Y124	AZFb	5′-CAGGCAGGACAGCTTAAAAG-3′
				5′-ACTGTGGCAAAGTTGCTTTC-3′
18	228 bp	Y128	AZFb	5′-GGATGAGACATTTTTGTGGG-3′
				5′-AGCCCAATGTAAACTGGACA-3′
19	125 bp	Y152	AZFd	5′-AAGACAGTCTGCCATGTTTCA-3′
				5′-ACAGGAGGGTACTTAGCAGT-3′
20	472 bp	SRY (Y14)	Control	5′-GAATATTCCCGCTCTCCGGA-3′
				5′-GCTGGTGCTCCATTCTTGAG-3′
21	Variable	Y87	AZFa	5′-TCTGTTGCTTGAAAAGAGGG-3′
				5′-ACTGCAGGAAGAATCAGCTG-3′
22	Variable	Y90	AZFa	5′-CAGTGCCCCATAACACTTTC-3′
				5′-ATGGTAATACAGCAGCTCGC-3′
23	Variable	Y109	AZFb	5′-AGGAGATGTCAGGACTATCAGC-3′
				5′-TCCATCCAGCTGGTCATATT-3′
24	Variable	Y117	AZFb	5′-GTTGGTTCCATGCTCCATAC-3′
				5′-CAGGGAGAGAGCCTTTTACC-3′
25	Variable	Y155	AZFc	5′-ATTTTGCCTTGCATTGCTAG-3′
				5′-TTTTTAAGCCTGTGACCTGG-3′
26	Variable	Y146	AZFc	5′-ACAAAAATGTGGCTCAGGGA-3′
				5′-AAATAGTGTGCCCACCCAAA-3′
27	Variable	Y182	AZFa	5′-TCAGAAGTGAAACCCTGTATG-3′
				5′-GCATGTGACTCAAAGTATAAGC-3′
28	Variable	Y157	AZFc	5′-CTTAGGAAAAAGTGAAGCCG-3′
				5′-CCTGCTGTCAGCAAGATACA-3′
29	Variable	Y158	AZFc	5′-CTCAGAAGTCCTCCTAATAGTTCC-3′
				5′-ACAGTGGTTTGTAGCGGGTA-3′
30	Variable	Y276	AZFc	5′-CCTACCGCATCAGTGAATTTC-3′
				5′-TCTGTATGTGGAGTACACATGG-3′
31	Variable	Y283	AZFc	5′-CAGTGATACACTCGGACTTGTGTA-3′
				5′-GTTATTTGAAAAGCTACACGGG-3′
32	Variable	Y238	AZFc	5′-AACAAGTGAGTTCCACAGGG-3′
				5′-GCAAAGCAGCATTCAAAACA-3′
33	Variable	Y277	AZFc	5′-GGGTTTTGCCTGCATACGTAATTA-3′
				5′-CCTAAAAGCAATTCTAAACCTCCAG-3′
34	Variable	Y272	AZFc	5′-GGTGAGTCAAATTAGTCAATGTCC-3′
				5′-CCTTACCACAGGACAGAGGG-3′