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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2009 May 3;47(3):300–306. doi: 10.1016/j.freeradbiomed.2009.04.033

Figure 1.

Figure 1

Evidence of ROS in the Neurosensory Retina and Ciliary Body. DHE histochemical staining of ROS. A. Neurosensory retina of wild-type (Nrf2+/+; n=4) and Nrf2 deficient (Nrf2−/−; n=4) mouse shows minimal red label in the retina (R), photoreceptor layer of the retina (*), and retinal pigmented epithelium (RPE) when unstimulated (PBS treated). Increased DHE labeling in the retina of Nrf2+/+ (n=4) and Nrf2−/− (n=4) mice after LPS stimulation. Note marked increase in Nrf2−/− mouse retina. B Ciliary body processes of Nrf2+/+ and Nrf2−/− mice showed increase in DHE labeling after LPS treatment compared with PBS treatment. Note marked increase in Nrf2−/− mouse after LPS stimulation. The lowest panels show the hematoxylin staining in Nrf2+/+ mice. Bar = 100μm. (C, D) The graphs show the ratio of DHE fluorescent intensity to PBS-treated Nrf2+/+ mice. *p<0.05.