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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2009 May 3;47(3):300–306. doi: 10.1016/j.freeradbiomed.2009.04.033

Figure 2.

Figure 2

Decreased mRNA expression of antioxidant enzymes in the neurosensory retina 3 hours after LPS injection. RT-qPCR analysis of A) HO-1, B) NQO-1, and C) GCLM expression from retinas of wild-type (Nrf2+/+; n=5 each group) and Nrf2 deficient (Nrf2−/−; n=5 each group) mice that were given an intraperitoneal injection of 60 μg LPS. The graphs show the ratio of expression to PBS-treated Nrf2+/+ mice. Note the decreased expression of Nrf2 specific enzymes NQO-1 and GCLM in Nrf2+/+ mice. HO-1 expression is unchanged (p<0.09). On the other hand, basal expression of NQO-1, GCLM, and HO-1 was significantly greater in the retinas of Nrf2+/+ mice than Nrf2−/− mice. As expected, NQO-1 and GCLM expression were not affected by LPS injection in Nrf2−/− mice. In LPS-treated mice, the expression of HO-1 was significantly lower in Nrf2−/− compared to in Nrf2+/+. *p<0.05, **p<0.01.