Table 3.

Autodephosphorylation rates of Spo0F mutants

Substitution Position(s)	Amino Acid At Position				Fold Change From Wildtype
	‘14’	‘59’	‘89’	kdephos (min-1)a
Wildtype	Q	K	Y	0.0039b ± 0.0011	NA
‘59’	Q	E	Y	0.24 ± 0.01	+62
	Q	N	Y	0.093b ± 0.005	+24
‘89’	Q	K	E	0.031 ± 0.001	+7.9
	Q	K	L	0.015 ± 0.002	+3.8
	Q	K	H	0.0051± 0.0001	+1.3
‘59’/‘89’	Q	E	L	0.42 ± 0.04	+110
	Q	N	E	0.42 ± 0.05	+110
	Q	N	L	0.33 ± 0.02	+85
	Q	N	H	0.17 ± 0.01	+44
‘14’/‘59’/‘89’	S	E	L	0.49 ± 0.10	+130
	F	N	E	0.29 ± 0.03	+74
	H	N	H	0.13 ± 0.00	+33

^aMean ± standard deviation. Wildtype Spo0F was measured 4 times and each mutant Spo0F was measured 2-3 times.

^bRates reported by Zapf et al. (Zapf et al., 1998) were 0.0039 and 0.087 min^-1 for wildtype and ‘59’KN substitution, respectively.