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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Exp Hematol. 2009 Jul;37(7):814–823. doi: 10.1016/j.exphem.2009.03.005

Figure 2. DipA treatment of fresh lin- cells improves homing in submyeloablated hosts.

Figure 2

(A) Dot plots demonstrate the schema used to detect homed F1 donor lin- cells (CD45.1+/CD45.2+) in host Boy J (CD45.1+/CD45.2-) marrow. (B) Fresh F1 lin- cells were isolated, the cell pool was divided, and half of the cells were treated with 5 mM DipA for 15 minutes. Cells were washed and transplanted into BoyJ hosts and analyzed for the fraction of homed cells 20h after transplant. Homing of untreated vs. DipA-treated cells: 0 cGy, 3.3 ± vs. 0.3 vs. 3.6 ± 0.2%; 300 cGy, 2.3 ± 0.4 vs. 5.2 ± 0.6%; 550 cGy, 1.7 ± 0.4 vs. 4.8 ± 1.2%; 1100 cGy, 3.2 ± 0.6 vs. 6.5 ± 0.3%. N = 4-5 hosts/group. *, P ≤ 0.04 comparing DipA-treated to untreated cells for a given radiation dose.