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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Exp Hematol. 2009 Jul;37(7):814–823. doi: 10.1016/j.exphem.2009.03.005

Figure 6. Short-term culture leads to improved engraftment in submyeloablated hosts.

Figure 6

Lin- marrow cells were prepared from Bl/6 donors; a portion of the cells were immediately transplanted into 550 cGy-conditioned Boy J hosts (“fresh” group); the rest were cultured overnight prior to transplant (“O/N culture” group). N = 5 hosts each for fresh and cultured cells. Composite donor chimerism data from two independent experiments (N = 14) comprise the 72h culture group. Donor chimerism is shown 4-5 months post-transplant. *, P = 0.022 comparing fresh to O/N cultured lin- cells; †, P < 0.0001 comparing O/N cultured to 72h cultured lin- cells. (B) 20h homing assays of overnight-cultured lin- cells without and with DipA treatment was performed. DipA treatment resulted in a modest but not significant increase in homing efficiency.