Figure 3.
Specific activation of signal transduction pathways in chimeric receptor lines with various mutations of several key amino acid residues of FGFR1. (A-B) Western blot analysis of Erk1/2 and PLCγ1 phosphorylation in normal adult NSCs in response to FGF-2 (20 ng/ml). (C-D) Western blot analysis of NGF-induced Erk1/2 phosphorylation in the TF1 line and in the mutant TF1L422A or TF1Y766F chimeric lines. (E) Western blot analysis of NGF-induced PLCγ1 phosphorylation in the mutant TF1L422A or TF1Y766F chimeric lines.