Table 1.
Pre-adult stages | Adult Stages | ||
Exposed to short days to induce diapause development | Exposed to long days to induce direct development | Non-reproductive Fed only sugar |
Reproductive Fed sugar and protein (liver) |
First Instar Larvae | First Instar Larvae | Day 1 Males | Day 1 Males |
Second Instar Larvae | Second Instar Larvae | Day 1 Females | Day 1 Females |
Early Third Instar Larvae | Early Third Instar Larvae | Day 2 Males | Day 2 Males |
Late Third Instar Larvae | Late Third Instar Larvae | Day 2 Females | Day 2 Females |
Wandering Larvae | Wandering Larvae | Day 3 Males | Day 3 Males |
Pupae | Early Diapause Pupae (15 days) | Day 3 Females | Day 3 Females |
Early Pharate Adult | Mid-diapause Pupae (30 days) | Day 4 Males | Day 4 Males |
Mid-Pharate Adult (red eye stage) | Late Diapause Pupae (60 Days) | Day 4 Females | Day 4 Females |
Late Pharate Adult (black bristle stage) | Day 5 Males | Day 5 Males | |
Day 5 Females | Day 5 Females | ||
Day 7 Males | Day 7 Males | ||
Day 7 Females | Day 7 Females | ||
Day 9 Males | Day 9 Males | ||
Day 9 Females | Day 9 Females |
Flesh fly life stages that were individually RNA extracted and combined equally prior to cDNA library synthesis and normalization.