Table 2.
PCR primers and probes
| Genes | Sequences | Sizes (bp) |
| TLR-1 | (F) 5' CAG TGT CTG GTA CAC GCA TGG T 3' | 105 |
| (P) 5' (FAM) CAC ATG CTT TGC CAT CCA AAA TTA G (TARMA) 3' | ||
| (R) 5' TTT CAA AAA CCG TGT CTG TTA AGA GA 3' | ||
| TLR-2 | (F) 5' TGT GAA GAG TGA GTG GTG CAA GT 3' | 78 |
| (P) 5' (FAM) TGA ACT GGA CTT CTC CCA TTT CCG TCT TT (TARMA) 3' | ||
| (R) 5' ATG GCA GCA TCA TTG TTC TCA T 3' | ||
| TLR-3 | (F) 5' CCT GGT TTG TTA ATT GGA TTA ACG A 3' | 82 |
| (P) 5' (FAM) CAT ACC AAC ATC CCT GAG CT (MGB) 3' | ||
| (R) 5' TGA GGT GGA GTG TTG CAA AGG 3' | ||
| TLR-4 | (F) 5' CAC TCG ATG TCA TTC CAA AGT TAT TG 3' | 88 |
| (P) 5' (FAM) TAC TAA GTA ATG ACT GTC ATG AAA GCA GCA T (TARMA) 3' | ||
| (R) 5' AGA GTG CCC CCT TTA AAC AAA TT 3' | ||
| TLR-5 | (F) 5' TGC CTT GAA GCC TTC AGT TAT G 3' | 77 |
| (P) 5' (FAM) CCA GGG CAG GTG CTT ATC TGA CCT TAA CA (TARMA) 3' | ||
| (R) 5' CCA ACC ACC ACC ATG ATG AG 3' | ||
| TLR-6 | (F) 5' GAA GAA GAA CAA CCC TTT AGG ATA GC 3' | 88 |
| (P) 5' (FAM) TGC AAC ATC ATG ACC AAA GAC AAA GAA CCT (TARMA) 3' | ||
| (R) 5' AGG CAA ACA AAA TGG AAG CTT 3' | ||
| TLR-7 | (F) 5' TTA CCT GGA TGG AAA CCA GCT AC 3' | 72 |
| (P) 5' (FAM) AGA TAC CGC AGG GCC TCC CGC (TARMA) 3' | ||
| (R) 5' TCA AGG CTG AGA AGC TGT AAG CTA G 3' | ||
| TLR-8 | (F) 5' AGC GGA TCT GTA AGA GCT CCA TC 3' | 107 |
| (P) 5' (FAM) CCT GAC AAC CCG AAG GCA GAA GGC (TARMA) 3' | ||
| (R) 5' CCG TGA ATC ATT TTC AGT CAA GAC 3' | ||
| TLR-9 | (F) 5' GCA GTC AAT GGC TCC CAG TTC 3' | 116 |
| (P) 5' (FAM) CCC ACA ATA AGC T (MGB) 3' | ||
| (R) 5' GCG GTA GCT CCG TGA ATG AGT G 3' | ||
| TLR-10 | (F) 5' TTA TGA CAG CAG AGG GTG ATG C 3' | 152 |
| (P) 5' (FAM) TTG ACC CCA GCC ACA ACG ACA CTG (TARMA) 3' | ||
| (R) 5' CTG GAG TTG AAA AAG GAG GTT ATA GG 3' | ||
| CCR-1 | (F) 5' CCC AAT GGG AAT TCA CTC ACC A 3' | 200 |
| (P) 5' (FAM) CCT GCA GCC TTC ACT TTC CTC ACG AAA (TARMA) 3' | ||
| (R) 5' CAA ACG GAC AGC TTT GGA TT 3' | ||
| CCR-3 | (F) 5' CAC AGC AGA GCC GGA ACT C 3' | 75 |
| (P) 5' (FAM) TGT GTT TTA GGT CAG ATG CAG AAA AT (TARMA) 3' | ||
| (R) 5' CTC CTT GGT CCT TCC TCT TTA GG 3' | ||
| CCR-5 | (F) 5' ACT GCA AAA GGC TGA AGA GC 3' | 204 |
| (P) 5' (FAM) ACT GAC ATC TAC CTG CTC AAC CTG GCC A (TARMA) 3' | ||
| (R) 5' CGA TTG TCA GGA GGA TGA TG 3' | ||
| CCR-7 | (F) 5' ACG GAC GAT TAC ATC GGA GA 3' | 195 |
| (P) 5' (FAM) ACA CGA CCA GCC CAT TGC CCA GT (TARMA) 3' | ||
| (R) 5' GGT CAT GGT CTT GAG CCT CT 3' | ||
| FasL | (F) 5' ACC TCA AGG GGG ACT GTC TT 3' | 170 |
| (P) 5' (FAM) ACA TGG TTG TGA CCT GAG GAT TTA AGG GAT GG (TARMA) 3' | ||
| (R) 5' TTA GTT TCA CCG ATG GCT CA 3' | ||
| TRAIL | (F) 5' CCC CTG CTG GCA AGT CAA 3' | 68 |
| (P) 5' (FAM) TGG CAA CTC CGT CAG CTC GTT (TARMA) 3' | ||
| (R) 5' CCT CAG AGG TTC TCA AAA TCA TCT T 3' | ||
F, forward primers; R, reverse primers; P, Taqman probes
TLR, toll-like receptor; CCR, chemokine receptor; FasL, Fas ligand; TRAIL, Tumor necrosis factor (TNF)-related apoptosis-inducing ligand