Fig. 3.

Deletion of the PEST-like sequences did not alter the specific binding of the mutant Ca_Vβ₃ to the Ca_Vα₁ subunit. Determination of Ca_Vβ₃ binding to the AID of the Ca_V2.2 subunit. Capacity of the wild-type and mutant [³⁵S]-Ca_Vβ₃ subunits to interact with the fusion protein GST-AID_2.2 was assayed by SDS-PAGE and autoradiography. Translation represents the equivalent volume of in vitro translation of [³⁵S]-Ca_Vβ₃ used in the binding assays.