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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Biochimie. 2009 Apr 22;91(7):888–893. doi: 10.1016/j.biochi.2009.04.011

Figure 4.

Figure 4

Effect of ATP and Triton X-100 on guanylyl cyclase activity intracellular domain variants. A) In vitro guanylyl cyclase activities for GCACC+GC (squares) and GCAID (circles) were monitored in the presence of 4mM MnCl2 (closed symbols) or 10mM MgCl2 (open symbols) with different concentrations of ATP. Enzymes were incubated with all the reaction components except GTP for 10min on ice. Catalysis was initiated by addition of GTP and allowed to continue for 10min at 37°C. Activity values are shown as percentage of activity obtained in the absence of ATP. Data presented is the representative of two experiments. B) Purified GCACC+GC (white bars) and GCAID (grey bars) were subjected to in vitro guanylyl cyclase assay in the presence of 5mM MgCl2, 2mM MnCl2 or 2mM Mncl2 and 0.1% triton X-100. Values shown are averages of three experiments each measurement carried out in duplicates.