Figure 4.

A) Both DC subsets can present (OVA)-1. CD11c⁺ DCs were stained with antibody against CD8α and I-A^b MHC class II molecules and sorted into their respective CD8α⁺ and CD8α⁻ populations. 2 × 10⁴ Cells of each subtype were incubated with 1 × 10⁵ purified OT-II T cells with graded doses of OVA or (OVA)-1 for 84 h; [³H]thymidine (1 μCi) was added for the last 12 h. All data points were performed in triplicate. B) Nonsaccharide 1 does not inhibit or potentiate pro-inflammatory signalling networks relative to unmodified OVA. 10⁵ Purified CD11c⁺ splenic DCs were incubated with media, OVA or (OVA)-1 at two different doses ±various TLR stimuli for 24 h. Supernatants were collected and analyzed for IL-10. LPS was used at 1 μg mL⁻¹; poly(IC), 25 μg mL⁻¹; Sendai Virus, 40 hemagglutination units (H.U.) mL⁻¹; CpG1826, 2 mm. C) IL-6, as in (B). D) IFN-γ, as in (B). Values represent the mean of duplicate measurements.