Expression analyses by semiquantitative RT-PCR of seven genes belonging to cluster 2 (Cl. 2), cluster 3 (Cl. 3), and cluster 8 (Cl. 8). Four different stages were assayed in each of the three DH lines: uninucleated microspores before stress treatment (UM), microspores after 4 days of stress treatment (s-4d), microspores after 4 days of culture (c-4d), and young pollen grains (P). Contig3116_at was used as control