Fig. 2.

a Schematic representation of the hGX sPLA2 protein containing the R38C polymorphism in the propeptide region. The arginine doublet preceding the mature protein sequence is indicated in bold. The arrow indicates the allelic substitution, and the amino acid change (arginine R to cysteine C) is indicated in red. b Immunofluorescence localization of hGX sPLA2 in COS-7 cells transfected with vectors encoding the R38 and the C38 proteins. The hGX sPLA2 protein is labeled with red. Nucleus is labeled in blue with DAPI. Confocal analysis was performed on a Leica SP2-AOBS confocal microscope as described in “Materials and methods”. Magnification: 50