Fig. 5.

Effect of temperature cell growth on the expression of hGX sPLA2. COS-7 cells transfected with either R38- or C38-containing expression vectors were grown at 30°C or 37°C for 72 h. The total amount of hGX sPLA2 protein was then quantified in the cell lysate (a) and supernatant (b) using the TR-FIA assay as described in “Materials and methods”. c COS-7 cells transfected with either the R38- or C38-containing or empty expression vectors were grown at 30°C or 37°C for 72 h, and hGX sPLA2 activity was measured in cell supernatants as described in “Materials and methods”. Cells transfected with the empty vector showed no detectable hGX sPLA2 protein or enzyme activity (not shown)