Table 2.

PCR characterization of bfl1 mutant and its putative revertants (PR)

Plant	Ac PCR	Ds PCR	PCR1a	PCR2a	PCR3a	Genotype of Ds insertion	Phenotype
bfl1	+	+	-	+	+	Ds/Ds	Mutant
PR1	+	-	-	+	-	Ds/Ds	Mutant
PR2	+	+	+	-	-	+/+	Normal
PR3	+	+	-	+	+	Ds/Ds	Mutant
PR4	+	+	-	+	+	Ds/Ds	Mutant
PR5	-	+	+	-	-	+/+	Normal
PR6	+	+	-	+	+	Ds/Ds	Mutant
PR7	+	+	+	+	+	Ds/+	Normal
PR8	+	+	-	+	+	Ds/Ds	Mutant
PR9	+	+	-	+	+	Ds/Ds	Mutant
PR10	-	+	-	+	+	Ds/Ds	Mutant
PR12	ND	+	+	-	-	+/+	Normal
WT	-	-	+	-	-	+/+	Normal

a): PCR1, PCR2 and PCR3 were done with primers LW1125_For and LW1125_Rev, LW1125_Rev and Ds5_112^-, and LW1125_For and Ds3_6587⁺, respectively.