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. 2009 Jun 8;106(25):10338–10342. doi: 10.1073/pnas.0901249106

Fig. 2.

Fig. 2.

The ABCG2 Q141K mutation results in reduced urate transport. (A) Across-species comparison of the ABCG2 protein sequence. Sequence is also compared with the cystic fibrosis transmembrane conductance regulator (CFTR). Note the close proximity of Q141 in ABCG2 to the mutational hot spot F508 in CFTR. (B) Western blot of oocytes expressing ABCG2 WT or Q141K (monomers and dimers of ABCG2 are detected). Actin was used as a loading control. (C) Accumulation rates in oocytes expressing ABCG2 WT or Q141K normalized to ABCG2 expression level. (D) Efflux rates for ABCG2 WT and Q141K. (WT [red triangles]: slope = 0.01, N = 55; Q141K [black squares]: slope = 0.02, N = 55). Mean ± SEM; **, P < 0.001.