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. 2009 Jun 9;106(25):10266–10271. doi: 10.1073/pnas.0811119106

Fig. 3.

Fig. 3.

Abnormal development of iNKT cells in Dicerfl/flTie2cre+ mice. (A) CD1d expression on CD4CD8 DP thymocytes and total splenocytes from Dicerfl/flTie2cre+ and littermates were compared. CD1d MFI is shown in parentheses. Data are representative of 3 independent experiments. (B) CD44 and NK1.1 expression in thymic iNKT cells. Events shown are gated on TCRβ and CD1d tetramer double-positive cells. (C) Absolute number of iNKT cell subpopulations (mean ± SD) based on their CD44 and NK1.1 expression patterns. (D–F) The iNKT cell subset distribution in thymus and spleen are abnormal in Dicerfl/flTie2cre+ mice. TCRβ and CD1d tetramer double-positive cells were analyzed in the thymus (D and E) and spleen (F) of Dicerfl/flTie2cre+ and littermates and the relative frequencies of subsets defined by CD4 and CD8 expression were assessed (mean ± SD).