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Gene expression methods used before (and after) GFP. (A) Positions of the six touch receptor neurons in C. elegans. (B) Antibody staining to the MEC-7 β-tubulin. [Reprinted with permission from ref. 13 (Copyright 1994, Company of Biologists, Ltd.)] (C) β-Galactosidase expression of a transcriptional fusion for mec-9. (Reprinted from ref. 14 with permission from Elsevier Limited.) (D) In situ hybridization to mec-7 mRNA (image by Shohei Mitani).
