Fig. 3.

A GluR6-derived LBD gates delta2 channels. (A) Typical current responses recorded upon application of Glu and KA before and after ConA treatment in NFR. The respective mean current responses (numbers of oocytes are indicated in the diagram) are shown in a bar diagram to the Right. (B) Typical current responses elicited by 300 μM Glu, 150 μM KA, 10 μM DA, and 10 μM AMPA from oocytes expressing delta2-(LBD)GluR6. To the Right, the respective mean agonist-induced responses (± SEM) of 5–22 oocytes are shown. The responses were recorded in at least 2 independent experiments in Ca²⁺-free MgR after treating the oocytes with ConA. (C) Dose–response curves for delta2-(LBD)GluR6 (black squares) in comparison to GluR6 wild type (white circles) recorded for Glu; each curve averaged from 5 oocytes. (D) Mean block (± SEM) of agonist-induced current responses by 300 μM GYKI 52466, and respective current traces in (E). Note that GYKI does block delta2-(LBD)GluR6 channels to 26 ± 2%.