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. 2009 Jun 5;106(25):10183–10188. doi: 10.1073/pnas.0812105106

Fig. 3.

Fig. 3.

Interaction of GRK2 and PTCH1 is critical for cyclin B1-mediated cell proliferation. (A–C) Coexpression of GRK2, K220R, NLS-cyclin B1 and BP attenuates the inhibitory effect of PTCH1 on cyclin B1-mediated stimulation of cell proliferation. Growth curves (A–B) and day 4 cell counts (C) of HEK293 cells transfected with the indicated constructs. Distinct colors indicate different groups according to the symbol types of A. (D–E) Expression of GRK2 rescues G2/M arrest in HEK293 cells. Cells were incorporated with BrdU 40–48 h after transfection and labeled with anti-p-H3 and anti-BrdU antibodies. Numbers of p-H3-positive cells (D) or BrdU-positive cells (E) were counted and percentages relative to the total number of mitotic cells (Total p-H3-positive cells plus BrdU-positive cells) were calculated. (F) miR-GRK2 induces cell cycle arrest in G2/M stage in HEK293 cells. Cells were transfected with miR-GRK2 or control siRNA. The average value of 3 independent experiments is shown. Error bars represent the standard error of the mean. *, P < 0.05 in pair-wise Student's test; **, P < 0.01 in pair-wise Student's t test.