Figure 4. The NLS of ErbB1 is important for ErbB1 nucleolin interaction.

(A) Schematic presentation of the chimeric protein expression vectors. (B) Cells were transfected with nucleolin-myc and either pEGFP (empty vector), pEGFP-TM-NLS or pEGFP-TM expression vectors. The resulting cell lysates were subjected to fractionation and the pellets were subjected to immunoprecipitation with anti-Myc antibodies and immunoblotted with anti GFP antibodies. As control, total cell lysates were blotted with anti-GFP and anti-Myc antibodies.