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. 2009 Jul 3;4(7):e6128. doi: 10.1371/journal.pone.0006128

Figure 6. The 212 C-terminal aa of nucleolin are sufficient to induce ErbB1 phosphorylation and dimerization.

Figure 6

(A) Cells were transiently co-transfected with expression vector of ErbB1 receptor and with either pEGFP, pEGFP-nucleolin-Myc (GFP-NCL) or pEGFP-212-nucleolin-Myc (GFP-212) mutants. Following 20 minutes serum deprivation, cells were treated with or without EGF 100 ng/ml for 10 minutes. Cell lysates were immunobloted with monoclonal antibody PY20. As a control lysates were immunoblotted with polyclonal antibody anti ErbB1 as indicated. (B) Cells were transiently co-transfected with expression vector of ErbB1 receptor and with either pEGFP, pEGFP-nucleolin-Myc (GFP-NCL) or pEGFP-212-nucleolin-myc (GFP-212) mutants. Following 20 minutes serum deprivation, cells were treated with or without EGF 100 ng/ml for 10 minutes. Cell lysates were incubated with BS3 cross linker and immunobloted with polyclonal antibody anti ErbB1.