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. 2009 Jul 3;4(7):e6128. doi: 10.1371/journal.pone.0006128

Figure 8. Nucleolin-212-C-ter mutant and ErbB1- induced anchorage-independent growth.

Figure 8

(A) HEK-293 cells stably expressing empty vector (not shown), ErbB1, nucleolin-212-C-ter or ErbB1 and nucleolin-212-C-ter were seeded in soft agar (4000 cells/well in 96 well plates) in medium containing 10% FBS, 0.3% agar, in the presence of 100 ng/ml EGF. The extent colony formation was determined 2 weeks later. Cells were dyed with MTT and the wells were photographed and colonies were counted. Representative image is presented. (B) Results were quantified using image analyzer program Image pro-Plus. The results are presented as the number of colonies at size larger then 0.01 mm2. Note that nucleolin-212-C-ter+ErbB1 but not each protein alone induced significantly more colonies (p<0.001). Results are the mean ± SD 6 determinations from each clone (3 clones from each stable cell line). Each experiment was repeated twice with similar results.