Fig. 1.
Adipose-specific rictor knockout mice have increased body weight. (A) Adipose-specific rictor knockout mice (rictorad−/−) were generated using the Cre/LoxP system (see Methods). (B) Immunoblot showing knockout of rictor and impaired mTORC2 signaling in adipose tissue of 2 littermates. A short (Right) and long (Left) exposure are shown. Residual rictor protein in rictorad−/− in the long exposure is likely from stromal vascular cells in the adipose tissue that do not express aP2-Cre. (C) Weight curves of rictorfl/fl (n = 16) and rictorad−/− (n = 14) male mice 8–18 weeks of age maintained on a chow diet. (D) Weight curves of rictorfl/fl and rictorad−/− male mice fed an HFD for 10 weeks (n = 10 per genotype). (E) Body length determined by measuring nasal-to-anal distance. Mice were put on an HFD at the age of 8 weeks. Values in C–E are mean ± SEM. *P < 0.05; **P < 0.01, rictorad−/− vs. rictorfl/fl.