Fig. 3.
OmpD is an important target of the humoral immune response to STm. (A) Nonimmunized (NI) or porin-immunized WT mice were infected with 5 × 106 STm (closed circles) or 5 × 106 STm lacking OmpD (STmOmpD− strain RAK126; open circles) for 5 days and splenic, liver and blood bacterial numbers enumerated (representative of 3 experiments for spleen and liver). Immunoblot analysis of IgM from NI or porin-immunized TCRβδ−/− mice against cell walls from STm or STm lacking OmpD. The boxed region shows the approximate Mr of the porins OmpC and F. (B) NI or porin-immunized WT mice were infected with 5 × 106 STm 5 × 106 STm cells (filled circles) or 5 × 106 STm cells lacking OmpR (STmOmpR− strain RAK83) (open circles) for 5 days and splenic, liver, and blood bacterial numbers enumerated (representative of 2 experiments). Immunoblot analysis of IgM from NI or porin-immunized TCRβδ−/− mice against cell walls from STm or STm lacking OmpR. The boxed region shows the approximate Mr of the porin OmpD. The Mann–Whitney test was applied to compare the statistical significance between groups linked by bars. *, P < 0.05; NS, not significant; d, day.