Skip to main content
. 2009 Jun 2;106(24):9785–9790. doi: 10.1073/pnas.0902844106

Fig. 3.

Fig. 3.

Reduced granzyme-B production in the absence of AHNAK1. (A) The expression of granzyme B was examined in wild-type and Ahnak1−/−-naive CD8+ T cells versus CTLs. (B) Kinetics of granzyme-B expression were obtained as in Fig. S1B. β-actin was used as the loading control. We performed 3 independent experiments. There is a statistically significant difference in granzyme-B expression in wild-type and Ahnak1−/− cells on the fifth day after primary stimulation (*, P < 0.05). Experiment B was performed in addition to and is independent of experiment A. (C) The granzyme-B ELISPOT assay was performed using wild-type or Ahnak1−/− CTLs, stimulated for 5 days as described in Fig. S1. We performed 3 independent experiments. There is a statistically significant difference in granzyme-B secretion in wild-type and Ahnak1−/− cells (P < 0.05).