Table 2.
Characteristic daughter ions obtained from MS/MS of the phosphopeptide F16Kp.
| Residue* | b | Intensity | b-H3PO4 | Intensity | y | Intensity | y-H3PO4 | Intensity |
|---|---|---|---|---|---|---|---|---|
| F | - | - | - | - | - | - | 1963.52 | 76000 |
| Q | - | - | - | - | 1914.70 | 913 | 1817.90 | 982 |
| [pS] | - | - | - | - | 1786.81 | 1825 | 1688.48 | 2407 |
| E | - | - | 474.07 | 197 | 1619.80 | 3272 | - | - |
| E | 701.21 | 240 | 603.07 | 258 | 1490.72 | 3184 | - | - |
| Q | 829.35 | 506 | 731.34 | 358 | 1361.71 | 3930 | - | - |
| Q | 957.46 | 842 | 859.44 | 1443 | 1233.66 | 5914 | - | - |
| Q | 1085.50 | 932 | 987.51 | 1216 | 1105.60 | 6820 | - | - |
| T | 1186.57 | 618 | 1088.58 | 2033 | 977.53 | 8903 | - | - |
| E | 1315.59 | 732 | 1217.62 | 1716 | 876.48 | 3832 | - | - |
| D | 1430.62 | 1490 | 1332.64 | 1394 | 747.42 | 3670 | - | - |
| E | 1559.30 | 763 | 1461.72 | 985 | 632.39 | 3402 | - | - |
| L | 1673.38 | 111 | 1574.77 | 881 | 503.33 | 775 | - | - |
| Q | 1800.00 | 605 | - | - | 390.23 | 324 | - | - |
| D | 1915.39 | 913 | 1817.90 | 982 | - | - | - | - |
| K | - | - | 1945.99 | 3805 | - | - | - | - |
*
[pS] indicated the phosphoserine residue.