Table 1.
Plant tissues | Specific activity, milliunits/ mg of protein
|
||
---|---|---|---|
+ADPG | +ADPG+ PPi | +ADPG+ PPi+3-PGA | |
Monocotyledonous | |||
Barley leaf | 113.7 ± 3.5 | 72.2 ± 0.7 | 103.8 ± 5.0 |
Barley leaf endosperm, 30 days after pollination | 2.0 ± 0.2 | 15.9 ± 1.2 | 21.8 ± 0.3 |
Wheat leaf | 22.4 ± 2.5 | 15.1 ± 0.9 | 50.8 ± 0.6 |
Wheat leaf endosperm, 30 days after pollination | 1.7 ± 0.2 | 48.3 ± 2.6 | 56.4 ± 1.4 |
Dicotyledonous | |||
A. thaliana leaf, Wt | 5.2 ± 0.6 | 5.1 ± 0.8 | 42.1 ± 3.3 |
A. thaliana leaf, Adg | 5.9 ± 1.7 | 6.5 ± 1.3 | 7.2 ± 0.2 |
Pepper leaf | 5.0 ± 0.6 | 7.3 ± 1.0 | 24.8 ± 0.8 |
Tomato leaf | 5.6 ± 0.5 | 16.1 ± 0.1 | 82.4 ± 6.9 |
Sycamore cultured cells | 16.5 ± 7.2 | 30.6 ± 5.4 | 137.4 ± 39.8 |
Data are presented as the mean ± SD obtained from five independent experiments. Details of enzyme assay methods to measure G1P production are described in Material and Methods.