Table 2.

Purification of AGPPase from young barley leaves

Step	Total volume, ml	Total protein, mg	Total activity, milliunits	Specific activity, milliunits/mg of protein	Purification, -fold	Yield, %
Crude extract	560.0	5107.8	105,000	20.6	—	100.0
Supernatant, 100,000 × g	520.0	3436.7	100,500	29.2	1.4	95.7
Ammonium sulfate, 50%	520.0	748.6	97,500	130.2	6.3	92.8
pH 4.2/62°C	520.0	24.9	90,500	3,634.0	176.4	86.2
Ammonium sulfate, 50%	5.7	8.1	47,300	5,839.0	283.4	45.0
Superdex 200	1.7	1.3	30,200	23,230.0	1127.6	28.7