Table 3.
Substrate specificity studies on barley AGPPase
| Substrates | Km, mM | Vmax, % with respect to ADPG | Products | Stoichiometry |
|---|---|---|---|---|
| ADPG | 0.5 | 100 | G1P + AMP | (1:1) |
| CDPglucose | 2.8 | 114 | G1P + CMP | (1:1) |
| UDPG | 2.1 | 114 | G1P + UMP | (1:1) |
| ADPribose | 2.4 | 100 | Ribose 5P + AMP | (1:1) |
| bis-PNPP | 0.3 | 100 | PNP + PNPP | (1:1) |
| NAD+ | 2.5 | 100 | AMP + NMN | (1:1) |
| NADP+ | NQ | NQ | — | — |
| cAMP | NQ | NQ | — | — |
| ATP | NQ | NQ | — | — |
| ADP | NQ | NQ | — | — |
Kinetic parameters (Km, Vmax, Ki, and stoichiometry) obtained are the mean values from five independent experiments. NQ, not quantifiable.